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These constructions concomitantly express 19-bp, 9-nucleotide stem-loop shRNAs with GFP, thus allowing the identification of transfected cells by fluorescence microscopy and flow cytometry
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Therefore, we also used the oncohistone H3.3K36M mutant, which has a dominant-negative effect on NSD family and SETD2 methyltransferases and results in depletion of H3K36me2 and H3K36me3 (refs
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