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The concept of using nanopore sensors for sequencing DNA was hypothesized in the 1980s through the identification of -hemolysin as a nanopore able to recognize ionic current blockades [90], and currently nanopore sequencing developed by Oxford Nanopore Technologies (ONT) represents the fourth-generation sequencing technique which is able to rapidly analyze long sequences of DNA without the prior need of PCR amplification [13], as well as to analyze DNA, RNA [84], or even drugs, polymers and macromolecules [91] (see Fig

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