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coli MG1655 (DE3) wet cells at boiling water bath for 30 min, and measured using an Agilent 1200 HPLC equipped with a ZORBAX SB-Aq column (4.6 × 150 mm 5 μM, Agilent) and interfaced with a Bruker microQ-TOF II mass spectrometer or a Waters ACQUITY UPLC H-Class system equipped with a PDA diode array detector, QDA mass spectrometry detector and a XBridge BEH C18 column (3.0 × 100 mm 2.5 μM)
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