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It ensures not only individual fitness but also the perpetuation and propagation of genetic materials across generations ( Some FDA (Food and Drug Administration) approved drugs for certain medical conditions, particularly antihypertension, have been implicated in male reproductive dysfunction due to their adverse effects on spermatogenesis and gonadal structure ( Antioxidants have been reported to play a crucial role in mitigating the harmful effects of oxidative stress on cells ( Allium savitum ), on the other hand, contains several sulfur-containing bioactive compounds notably allicin (a lipophilic bioactive compound) and S-allyl cysteine (a hydrophilic compound), which exhibit the antioxidant, anti-inflammatory, and immunomodulatory properties ( Synergistic effects are expected when vitamin C and Allium sativum are administered together

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Briefly, 1.5 μg of pEGFP-GST-GPI BY55 , pEGFP-GST-GPI DAF , pEGFP-GST-GPI CEAM7 , pEGFP-GST-TM ITB3 , pEGFP-GST-TM ITA5 and pEGFP-GST-TM ITAV plasmids were transfected using 3 μL of 1 mg/ml PEI as described above