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Frontiers | Mechanism of homocysteine-mediated endothelial injury and its consequences for atherosclerosis

Lysates containing chromatin fragments were incubated with washed antibody-bound beads for overnight at 4°C and washed with low-salt wash buffer [1% TritonX-100, 0.1% SDS, 2 mM EDTA (pH8.0), 150 mM NaCl, 20 mM Tris-HCl (pH8.0)], high salt wash buffer [1% TritonX-100, 0.1% SDS, 2 mM EDTA (pH8.0), 500 mM NaCl, 20 mM Tris-HCl (pH 8.0)] and LiCl buffer [0.25 M LiCl, 1% NP-40, 1% NaDOC, 1 mM EDTA, 10 mM Tris-HCl (pH 8.0)] with tube exchange at each steps

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Size:Pack of 30

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