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This blinding process was implemented to eliminate potential bias in the scoring of tissue samples, ensuring objective evaluation of tubular damage across all experimental conditions Biochemical analysis The levels of malondialdehyde (MDA) and the activities of adenosine triphosphatase (ATP) and manganese superoxide dismutase (MnSOD) in kidney tissues were measured using the MDA Assay Kit, ATP Assay Kit, and SOD Assay Kit, respectively (all from Abcam)
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