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Consistently, central and peripheral injections of ghrelin in obese mice fail to induce AgRP and NPY expression and secretion 221,224 , raising the possibil that impaired response of AgRP-expressing and NPY-expressing neurons contributes to ghrelin resistance in the hypothalamus
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coli using a glutathione S-transferase (GST) fusion system is a method of choice for studying the function of particular proteins, for purifying interacting factors and for generating antibodies against fused proteins
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