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Volume:30ml
Cell lysate for quantification of GSH, GSSG, protein thiols and disulfides The cell pellet was washed with 5 mL ice-cold PBS with 1 mM EDTA, suspended in 0.5 mL of 10% sulfosalicylic acid, and sonicated using a Misonix XL2020 sonicator with a cup horn probe (Farmingdale, NY) for 4 min
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