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Kinetic fluorescence measurements were performed in a microplate reader at an excitation wavelength (λex) of 485 nm and an emission wavelength (λem) of 530 nm, with a runtime of 15 min at 1-min intervals and a temperature set to 25 °C to determine the average velocity (Vmean) of the enzymatic reaction

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The final distance from the lens to the film plane was somewhere around 30mm (obviously, since the lens has a focal distance of 30mm), which meant that the lenses are recessed into the camera body by about 10mm