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A replication study published in 2025 in the journal Biogerontology (Al-Dulaimi et al., 2025) has now independently revisited the central finding

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Feuerstein, an associate professor of medicine at Harvard Medical School and clinical chief of gastroenterology at Beth Israel Deaconess Medical Center, says that applying methylene blue to the colon epithelium during a colonoscopy creates a "great contrast, where you can actually see it enhance the detection of polyps, and precancerous growths in the colon in general." He uses it mostly in patients with longstanding inflammatory bowel disease (IBD) because of their increased risk for colorectal cancer Besides aiding in the detection of multiple types of cancerous and precancerous lesions, methylene blue is sometimes combined with light therapy (such as that from a laser) to help treat lung cancer, breast cancer, and others