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[DOI] [PMC free article] [PubMed] [Google Scholar] 13.Zhu J.L., Cai Y.Q., Long S.L., Chen Z., Mo Z.C
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Originally shown to bind only one SAM molecule per monomer, more recent ITC studies suggested a two-site model where a kinetically stabilising high-affinity SAM binding site would be formed from oligomerisation, while enzyme activation is driven by SAM binding to the lower affinity S2 site observed in dimeric CBS Δ516–525 crystal structures 23,28,35
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