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For F-actin and cellular membrane costaining, cells were incubated with deep red actin tracking stain dye (Thermo, A57245) and CellMask green plasma membrane stain dye (Thermo, C37608) at room temperature in the dark for 30 min, washed with 1× PBS and mounted with DAPI-free mounting medium (Beyotime, P0126)

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Thus, Ziska (2022) acknowledges that breeding can change nutritional density but ultimately concludes that “[CO2] is directly affecting protein concentration separate from breeding history”