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Cellular extraction for biochemical assays Cells were washed two times with cold PBS and lysed in a buffer containing 50 mM Tris (hydroxymethyl) aminomethane hydrochloride (pH 7.4), 150 mM sodium chloride, 1% Triton X-100, and protease inhibitors
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Size:70gm
The most widely used detection method involves the derivatization with 2,4-dinitrophenylhydrazine (DNPH) and spectrophotometric quantification at 370–375 nm, with the absorbance being proportional to carbonyl content
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