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Resin-bound RABs were equilibrated in a buffer (25 mM HEPES pH 7.5, 110 mM KCl, 1 mM DTT, 10 mM EDTA) with 1.25 mM GDP or GTPγS (Jena biosciences #UN-1172 and #UN-412) for 30 min at 30°C
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