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Cells were lysed in ice-cold RIPA buffer (1% NP40, 0.1% SDS, 0.5% deoxycholate in PBS, supplemented with 1 mM PMSF, 1X cOmplete™, EDTA-free Mini Protease Inhibitor Cocktail (Sigma Cat#11873580001) and 1X PhosSTOP™ (Sigma Cat#4906845001)) for 15 minutes
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