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Future studies could incorporate high-dimensional omics and AI analytics into zPDX platforms, forming an intelligent feedback loop of “clinical sample → zebrafish validation → efficacy prediction.” Although existing studies confirm that human cells can survive short-term and retain functionality in zebrafish embryos or larvae, and although transplantations of human tumor fragments or immune cells have yielded encouraging results in drug sensitivity and inflammation studies (Wang L

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Espinosa et al., 2013)