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Selenium and hydrogen selenide: essential micronutrient and the fourth gasotransmitter? | Intensive Care Medicine Experimental | Springer Nature Link

Additionally, there is a common “E-flap” motif in GS enzymes which is a conformationally variable loop that contains a catalytic glutamate at loop turn that is presumed to deprotonate ammonium ions to generate active ammonia (Figure 1)

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Briefly, standards were first treated with 5 volumes of SSA solution and then further diluted 500 fold with the phosphate-EDTA buffer (pH 7.4)