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Abs
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Appropriate sample was taken into a 1.5 mL centrifuge tube, add 500 μL water and add 100 mg glass beads in 1.5 mL centrifuge tube, homogenized for 1 min and centrifuged at 12,000 rpm at 4 °C for 10 min, and 200 μL of the supernatant was collected and 100 μL of 15% phosphoric acid and 20 μL of 375 μg/mL internal standard (4-methylvaleric acid) solution were added, followed by mixing with 280 μL of ether for 1 min, the mixture was then centrifuged at 12,000 rpm at 4 °C for 10 min, and the supernatant was tested on the machine
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