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Glutathione Restoration Protocol Foundation Protocol (daily): - NAC: 600 mg twice daily on an empty stomach - Glycine: 3-5 g/day (can be taken before bed — improves sleep quality) - Selenium (selenomethionine): 200 mcg/day — cofactor for glutathione peroxidase - Vitamin C: 1000 mg twice daily — recycles oxidized GSSG back to GSH - Alpha-lipoic acid (R-form): 300-600 mg/day — universal antioxidant that regenerates GSH Advanced Protocol (confirmed deficiency): - Liposomal glutathione: 500-1000 mg in the morning on an empty stomach - Milk thistle (silymarin): 420 mg/day — stimulates GSH synthesis in hepatocytes (Hepatology, 2010) - Sulforaphane (broccoli extract): 50-100 mg/day — activates the Nrf2 pathway, upregulating glutathione synthesis genes - S-adenosylmethionine (SAMe): 400-800 mg/day — methyl donor, supports Phase II methylation reactions Diagnostic Assessment Erythrocyte glutathione (GSH/GSSG ratio) — the most accurate marker of intracellular stores - Organic Acids Test (OAT) — pyroglutamic acid is elevated in GSH deficiency - 8-OHdG — marker of oxidative DNA damage - Genetic testing — GSTM1, GSTT1, GSTP1, GPX1 polymorphisms determine individual requirements Frequently Asked Questions Can diet alone raise glutathione levels

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Nature 2022, 606 (7912), 49–58