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In addition to other regulatory mechanisms, such as tonic activity changes 2 , increased number of active neurons 53 and modulation at the nerve terminal level 54 , FFL motifs may thus provide a mechanism 55,56 to maintain hypothalamic circuit robustness in the face of perturbed metabolic homeostasis 57,58

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A list of all plasmids used to generate gene edited cell lines can be found in Supplementary Table 1