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Considering that the test is for aqueous solution, a calcium fluoride window sheet (applicable range 7,800–1,100 cm −1 , refractive index: 1.43) with high transmittance and being not easily deliquescent was chosen to be fixed in the liquid cell, and the IR spectra were collected before and after the enhancement of different concentrations of glutathione solutions by transmission

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This axis encompasses multiple pathways of information exchange—including neural signaling, endocrine regulation, and immune-mediated mechanisms—that together sustain functional communication between the gastrointestinal tract and the CNS (59)